Two simple, sensitive and accurate spectrophotometric methods for the determination of tizanidine hydrochloride have been developed. The methods are based on reaction of tizanidine hydrochloride with folin-ciocalteu reagent under basic condition and dissociation which reduces the folin-ciocalteu reagent giving a blue coloured solution (Method A), while tizanidine hydrochloride in presence of acidic medium reacts with excess amount of ceric ammonium sulfate and the unreacted ceric ammonium sulfate reacts with crystal violet to produce greenish blue colour. The final solutions were made to produce 100 µg/ml with distilled water for both method A and method B. The λmax was found to be at 723 nm and 448 nm and the linearity was found in concentration range of 10-50 μg/ml and 10-60 μg/ml respectively for method A and method B. The correlation coefficient was found to be 0.9979 and 0.9991 respectively for method A and method B. The methods were validated as per USP and ICH guidelines.
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